| 產(chǎn)品名稱(chēng) |
11-26c |
| 商品貨號(hào) |
B161716 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with all allotypes of mouse IgD and can be used in FACS and ELISA analysis of murine B cells. |
| Derivation |
Lymph node cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse IgD |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse IgD |
| Comments |
Animals were immunized with mouse IgD. Lymph node cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with all allotypes of mouse IgD and can be used in FACS and ELISA analysis of murine B cells. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal
bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG2a |
| Name of Depositor |
JF Kearney |
| Deposited As |
rat (B cell); mouse (myeloma) |
