| 產(chǎn)品名稱 | Bak KO SV40 MEF |
|---|---|
| 商品貨號 | B221824 |
| Organism | Mus musculus, mouse |
| Tissue | embryonic fibroblast |
| Cell Type | fibroblast |
| Product Format | frozen |
| Morphology | fibroblast-like |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain SV40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | This cell line is useful to study molecular mechanism of cell apoptosis, BCL-2 family pathway, BAK function, as well as cells that are resistant to multiple apoptotic stimuli. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | Cells are immortalized MEFs, which were generated from BAK knockout mice. |
| Tumorigenic | no |
| Comments | Cells are immortalized MEFs (genotype: bak-/-), which were generated from BAK knockout mice. Cells are sensitive to tBID-induced cytochrome c release and apoptosis. Cells are sensitive to multiple apoptotic stimuli that act through disruption of mitochondrial function: staurosporine, ultraviolet radiation, growth factor deprivation, etoposide, and the endoplasmic reticulum stress stimuli thapsigargin and tunicamycin. Cells are sensitive to Bim and Bad expression induced apoptosis. BAK is a pro-apoptotic BCL-2 family member. This cell line is useful for studying molecular mechanism of cell apoptosis, BCL-2 family pathway, BAK function, as well as cells that are resistant to multiple apoptotic stimuli. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated IMDM Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: 10 % Fetal Bovine Serum and 1x Non-essential amino acids.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
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| Cryopreservation | Freeze medium: 90% Fetal Bovine Serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor | Stanley Korsmeyer |
| References | Wei M, et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science 292(5517): 727-730, 2001 PubMed: 11326099 Lindsten T, et al. The Combined Functions of Proapoptotic Bcl-2 Family Members Bak and Bax Are Essential for Normal Development of Multiple Tissues. Molecular Cell 6(6): 1389-1399, 2000 PubMed: 11163212 Zong WX, et al. BH3-only proteins that bind pro-survival Bcl-2 family members fail to induce apoptosis in the absence of Bax and Bak. Genes Dev. 15(12): 1481-6, 2001 PubMed: 11410528 Cheng EH, et al. BCL-2, BCL-XL Sequester BH3 Domain-Only Molecules Preventing BAX- and BAK-Mediated Mitochondrial Apoptosis. Molecular Cell 8(3) : 705–711, 2001 PubMed: 11583631 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
