| 產品名稱 |
PVlambda1.6 |
| 商品貨號 |
B222515 |
| Designations |
PVlambda1.6 |
| GenBank Number |
J00590
|
| Species |
Mus musculus, mouse |
| Depositors |
U Storb |
| Applications |
Subcloned from Rlambda1, this has the same sequence as Ig303. The T7 promoter is used to make anti-sense RNA. |
| Vector |
Construct size (kb): 3.0 |
| Insert |
DNA: genomic Insert lengths(kb): 0.2520000040531158 Tissue: myeloma H2020 cell line Gene product: immunoglobulin lambda chain variable-1 [IglV1] |
| Insert Size (kb) |
0.252 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI/SstI--2.7, 0.25; EcoRI--3.0; KpnI--uncut. Subcloned from Rlambda1, this has the same sequence as Ig303. The T7 promoter is used to make anti-sense RNA. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Bernard O, et al. Sequences of mouse immunoglobulin light chain genes before and after somatic changes. Cell 15: 1133-1144, 1978. PubMed: 103630
Deanna Haasch, personal communication
|
