Vector permitting positive selection for integration
Vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; KpnI--3.6, 1.8; SstI--5.4.
Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts.
A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g. as a BamHI cassette).
When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector.
Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration.
The order of the major features of the plasmid is: gtmR - SstI/MCS/KpnI - P15A - traJ - oriT - sacB.
Growth Conditions
Temperature: 37°C
References
Quandt J, Hynes MF. Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Gene 127: 15-21, 1993. PubMed: 8486283
Shipped
frozen
Shipping Information
In glycerol stock of E. coli containing the plasmid
