| Vector |
Construct size (kb): 16.10000038146973 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBlueBac
Intact vector size: 14.000
Type of vector: phagemid
Vector end: NheI
Vector end: NheI
Cloning sites: NheI
Polylinker sites:
Host range: insect cells; Escherichia coli
Features (with orientation and position when available):
insert detection: lac
marker(s): ampR
promoter: polyhedrin
replicon: pMB1, f1, SV40
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCD
Gene name: protein kinase C, delta
Contains complete coding sequence?: Y
Localization: 3
Tissue: liver, HepG2 cell line
Type of DNA: cDNA
Insert end: Modification: NheI linkers
Insert end: Modification: NheI linkers
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: L07860 Insert lengths(kb): 2.099999904632568 Tissue: liver, HepG2 cell line Gene product: protein kinase C, delta [PRKCD] Target Gene: protein kinase C, delta |
| Comments |
Restriction digests of the clone give the following sizes (kb): NheI--14.0, 2.1; BglI--5.6, 5.4, 2.2, 1.8, 1.2; HindIII--8.8, 4.0, 1.3, 1.1, 1.0; NcoI--14.0, 1.25, 0.5, 0.35; PstI--14.0, 1.1, 0.55, 0.3. Insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.13; BglII--1.01; HindIII--0.85; NcoI--1.18, 1.64, 1.96; PstI--0.05, 0.33, 1.39; SalI--0.17. Contains the complete coding sequence, and begins at the initiation codon (nt 59 of the sequence record). |
