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Construct size (kb): 48.79999923706055

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon 4A
Intact vector size: 46.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GC
Genomic copy number: unique
Gene name: group-specific component (vitamin D binding protein)
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 q12-q13
Tissue: fetal liver (ATCC 37333)
Type of DNA: genomic
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert size (kb): 17.85
Cross references: DNA Seq. Acc.: L10641
Nucleotides ~1-17850 of the insert correspond to
nucleotides ~19800-37650 of L10641.

Insert lengths(kb): 17.85000038146973

Tissue: fetal liver (ATCC 37333)

Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) [GC]

Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11, 6.2, 3.5, 2.7, 2.5, 1.9, 1.15; HindIII--18 (doublet), 6.2, 2.4, 1.4, 1.1, 1, 0.4; BamHI--14 (doublet), 8, 6.2, 4, 1.5, 0.5; SstI-->23, 8.5, 4.2;

BglII--18, 5.2, 4.85, 4.7, 3.7, 2.2, 2.1, 1.9, 1.4, 1.0, 0.9, 0.8, 0.75, 0.65, 0.4, 0.3.

The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.9, 1.05, 2.95, 5.45, 11.65, 14.35; SacI--2.4; HindIII--8.1, 10.4, 11.5, 12.8, 13.8, 14.2.

Begins in intron 2 and extends into intron 11.

ATCC 95664/95665 and 95666/95667 overlap by 6.45 kb and share EcoRI fragments of the following sizes (kb): 0.15, 1.9, 2.5.

ATCC 95666/95667 and 95668/95669 overlap by 7.8 kb and share an EcoRI fragment of 2.7 kb.

Contains Alu repeats, Kpn repeats, and a THE repeat.