| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--3.8, 0.84, 0.37; BamHI--4.1, 0.82; SalI/HindIII--3.9, 0.66, 0.39; BamHI/HindIII--4.3, 0.6; ClaI--4.9; EcoRI--5.2, 0.5. MspI identifies 2 additional polymorphisms. Only insert DNA should be used for nick translation. Codominant segregation demonstrated in 4 informative families with 22 individuals. Enzyme(s) not detecting polymorphism: BamHI, BglII, HindIII, PvuII, XbaI with a panel of 9 unrelated Caucasians. |
