| 產品名稱 |
pMHZ13 |
| 商品貨號 |
B224105 |
| Designations |
pMHZ13 |
| Species |
Homo sapiens, human |
| Depositors |
RL White, Y Nakamura |
| Applications |
Otherwise, RFLPs observed under normal conditions of hybridization and washing. |
| Vector |
Construct size (kb): 6.300000190734863 |
| Insert |
DNA: genomic Insert lengths(kb): 3.299999952316284 Gene product: DNA Segment, single copy [D9S13] |
| Insert Size (kb) |
3.300 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): HincII--uncut; PvuII--6.3; EcoRI--5.4, 0.71; BamHI--6.3; HindIII--6.3. Codominant segregation of the PstI VNTR RFLP was demonstrated in 31 3-generation families. This probe should be preassociated with excess human DNA before hybridization. Otherwise, RFLPs observed under normal conditions of hybridization and washing. MspI does not resolve this polymorphism adequately. |
| References |
Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pMHZ13) on chromosome 9q [D9S13]. Nucleic Acids Res. 16: 5703, 1988. PubMed: 2898772
Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872
|
