Propagation in a dam- host is necessary for cleavage at the BclI site.
A positive selection vector for cloning Sau3AI-generated DNA fragments.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Burns DM, Beacham IR. Positive selector vectors: a small plasmid vector useful for the direct selection of Sau3A-generated overlapping DNA fragments. Gene 27: 323-325, 1984. PubMed: 6329908
