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Size (kb): 3.5840001106262210

Vector: pKSM718 (phagemid)

Promoters: Promoter for in vitro transcription T7lac

Construction: pKSM710, pUC18

Marker(s):ampR

Construct size (kb): 3.584000110626221

Features: insert detection: lacZalpha
marker(s): ampR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
MCS: DsaI...EcoRI
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB

expression vector

vector permitting RNA synthesis in vitro

vector permitting construction of fusion proteins

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): BamHI--3.6; EcoRI--3.6; BglI--3.6.

Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglI--3.29; EcoRI--0.39; NcoI--0.33; PvuI--0.51, 3.04; PvuII--0.48.

Differs from pKSM717 (ATCC 87162) only in the orientation of the ampicillin resistance gene.

Expression vector allowing generation of N-terminal deletions or modifications of foreign DNA cloned into the multiple cloning site.

Temperature: 37.0°C