| Derivation |
Animals were immunized with 3H5-1 (ATCC HB-46), a monoclonal antibody to Dengue 2 virus, conjugated to KLH (keyhole limpet hemocyanin). Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody binds specifically to an idiotypic determinant on 3H5 anti-Dengue 2 antibody. Dengue 2 virus inhibits the binding of 18-26-4 antibody to 3H5. The antibody reacts with sera from mice immunized to Dengue 2 virus and also with human antibody in acute and convalescent human dengue patient samples. |
| Comments |
Animals were immunized with 3H5-1 (ATCC HB-46), a monoclonal antibody to Dengue 2 virus, conjugated to KLH (keyhole limpet hemocyanin). Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody binds specifically to an idiotypic determinant on 3H5 anti-Dengue 2 antibody. Dengue 2 virus inhibits the binding of 18-26-4 antibody to 3H5. The antibody reacts with sera from mice immunized to Dengue 2 virus and also with human antibody in acute and convalescent human dengue patient samples. |
| References |
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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