| Vector |
Construct size (kb): 5.400000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-2
Intact vector size: 2.870
Type of vector: plasmid
Vector end: SalI
Vector end: SalI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->, 10-60
promoter for in vitro transcription: T7, <-, 65-87
replicon: pMB1
marker(s): ampR, <-, 1098-1958
promoter for in vitro transcription: SP6, ->, 2852-2869
Cross references: DNA Seq. Acc.: X65301 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: LPL
Other gene symbols: LIPD
Genomic copy number: unique
Gene name: lipoprotein lipase
Contains complete coding sequence?: Y
Chromosome: 8; Localization: 8 p22
Tissue: adipose
Type of DNA: cDNA
Insert end: Modification: SalI linker
Insert end: Modification: SalI linker
Insert size (kb): 2.435
Cross references: DNA Seq. Acc.: M15856
Nucleotides ~1-2425 of the insert correspond to
nucleotides 1-2425 of M15856. Insert lengths(kb): 2.434999942779541 Tissue: adipose Gene product: lipoprotein lipase [LPL] Alleles: Ca1, Ca2, A1, A2, A3, Ca1, Ca2, Da1, A1, A2, A3, Da1, Da2, Da2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): SalI--2.9, 2.5; EcoRI--4.5, 0.5, 0.35; XbaI--5.4; HindIII--5.4; PstI--5.4. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--1.64, 1.98; XbaI--2.26; PvuII--0.32, 0.73, 2.39. |
| References |
Wion KL, et al. Human lipoprotein lipase complementary DNA sequence. Science 235: 1638-1641, 1987. PubMed: 3823907
Michael C Schotz, personal communication
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