| Applications |
Only insert DNA should be used for nick translation. Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, MspI, PvuII, TaqI, XbaI. Tested against DNA from 9 unrelated Caucasians. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 0.6; PstI--5.4, 0.45; BamHI--5.8; PvuII--2.9, 2.9; SalI--5.8. Detects fragments of different chromosomal origin; polymorphic bands on chromosome 12, constant bands on chromosome 5, 12, 13, and 17. Codominant segregation demonstrated in 4 informative families with 20 individuals. Digest with BglII gives the following constant bands: 30, 18, 13, 9.6, 8.2, 3.5, 3, 2.6, 2. Only insert DNA should be used for nick translation. Genomic fragments should be separated on 0.9% gels. Washing conditions: 0.1X SSC at 63-65C. Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, MspI, PvuII, TaqI, XbaI. Tested against DNA from 9 unrelated Caucasians. |
