A lambda replacement vector for construction and screening of genomic libraries.
The left arm is 20 kb, the stuffer is 14 kb, and the right arm is 9 kb. lambdaEMBL3 (ATCC 37266) and lambdaEMBL4 (ATCC 37267) differ only in the orientation of the MCS surrounding the stuffer fragment.
References
Frischauf AM, et al. Lambda replacement vectors carrying polylinker sequences. J. Mol. Biol. 170: 827-842, 1983. PubMed: 6315951
