| 產(chǎn)品名稱 | CBRM1/10 |
|---|---|
| 商品貨號 | B233579 |
| Organism | Mus musculus, mouse |
| Tissue | spleen |
| Cell Type | hybridoma: lymphoblast B lymphocyte; somatic cell hybridoma |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain | BALB/c |
| Applications | The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact.
Ref  Diamond MS, et al. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J. Cell Biol. 120: 1031-1043, 1993. PubMed: 7679388 |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | Mice (BALB/c) were immunized with purified Mac1 (CD11b/CD18). Spleen cells were fused with P3X63Ag8.653 (ATCC CRL-1580). |
| Genes Expressed | immunoglobulin; monoclonal antibody against
C-terminal epitope of human Mac-1 |
| Cellular Products | immunoglobulin; monoclonal antibody against
C-terminal epitope of human Mac-1
|
| Comments | Monoclonal antibodies from selected hybridomas recognized the Mac-1 alpha subunits on the surface of neutrophils. The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact.
RefLu C, et al. The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. Dependence on beta subunit association and prediction of domains.. J. Biol. Chem. 273: 15138-15147, 1998. PubMed: 9614126 |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 104) to 1 X 10(5) viable cells/ ml. Maintain cultures at a cell concentration between 2 X 10(5) and 1 X 10(6) cells/ml. |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype | IgG1; Kappa light chain |
| Name of Depositor | TA Springer |
| Year of Origin | November 20, 1991 |
| References | Diamond MS, et al. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J. Cell Biol. 120: 1031-1043, 1993. PubMed: 7679388 Lu C, et al. The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. Dependence on beta subunit association and prediction of domains.. J. Biol. Chem. 273: 15138-15147, 1998. PubMed: 9614126 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
