Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
test tube
Storage Conditions
Test Tube: See handling procedure
Axenic/Xenic
Axenic
Type Strain
no
Comments
Oral morphogenesis
Prey-induced transformation
Medium
ATCC® Medium 1651: MA medium
Growth Conditions
Temperature: 25°C
Cryopreservation
Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min.
Adjust the concentration of cells to 2 x 106 - 2 x 107/ml in fresh medium.
While cells are centrifuging prepare a 22% (v/v) solution of sterile DMSO in fresh medium.
Mix the cell preparation and the 10% methanol in equal portions. Thus, the final concentration will be 106 - 107 cells/ml and 11% (v/v) DMSO. The time from the mixing of the cell preparation and methanol stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min.
Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.
Name of Depositor
AT Soldo, EB Small
Chain of Custody
ATCC <-- AT Soldo, EB Small <-- G. Holz
References
Gomez-Saladin E, Small EB. Oral morphogenesis of the microstome to macrostome transformation in Miamiensis avidus Strain Ma/2. J. Eukaryot. Microbiol. 40: 363-370, 1993.
Gomez-Saladin E, Small E. Prey induced transformation of Miamiensis avidus strain Ma/2 by a soluble factor. J. Eukaryot. Microbiol. 40: 550-556, 1993.
