| 產(chǎn)品名稱 | Human rhinovirus 31 |
|---|---|
| 商品貨號 | B236630 |
| Classification | Picornaviridae, Rhinovirus, Human rhinovirus A |
| Agent | Human rhinovirus 31 |
| Strain | 140 F |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | This item has been treated with diethyl ether to remove Mycoplasma orale at the ATCC. The previous item number was VR-506™ |
| Effect on Host | CPE, cell rounding and sloughing |
| Recommended Host | HeLa (ATCC® CCL-2™) |
| Growth Conditions | Temperature: 33°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.005 - 0.01 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, while rocking. End adsorption by adding virus growth medium. Incubation: 1-7 days at 33°C in a humidified 5% CO2 atmosphere while rocking, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor | VV Hamparian and RM Conant |
| Source | Human throat washings |
| References | Conant RM, and Hamparian VV. Rhinoviruses: Basis for a Numbering System. I. HeLa cells for propagation and serologic procedures. J Immunol 100 (1): 107-113, 1968. PubMed: 4295059 Conant RM, and Hamparian VV. Rhinoviruses: Basis for a Numbering System. II. Serologic characterization of prototype strains. J Immunol 100 (1): 114-119, 1968. PubMed: 4295060 Connelly AP Jr., Hamre D. Virologic studies on acture respiratory disease in young adults. II. Characteristics and serolotic studies of three new Rhinoviruses. J. Lab. Clin. Med. 63: 30-43, 1964. PubMed: 14102901 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
