| 產(chǎn)品名稱 |
RA-1 |
| 商品貨號 |
B237971 |
| Designations |
RA-1 |
| GenBank Number |
D16217
|
| Species |
Homo sapiens, human |
| Depositors |
H Menard |
| Applications |
produces protein calpastatin |
| Vector |
Construct size (kb): 5.919000148773193 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEX-4T-1
Intact vector size: 4.969
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SmaI SalI XhoI NotI
Polylinker sites: EcoRI SmaI SalI XhoI NotI
Other unique sites: PstI mLuI BstEII ApaI BssHII EcoRV NarI BamHI
Construction: pGEX-2T
Host range: Escherichia coli
Features (with orientation and position when available):
other: thrombin protease cleavage site
MCS: EcoRI/.../NotI
marker(s): ampR, ->
replicon: pMB1
repressor gene: lacI, ->
promoter: tac, ->
coding sequence: S. japonicum glutathione S-transferase
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CAST
Gene name: calpastatin
Contains complete coding sequence?: N
Chromosome: 5; Localization: 5 q15-q21
Tissue: placenta
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.95
Cross references: DNA Seq. Acc.: D16217
Nucleotides 1-950 of the insert correspond to
nucleotides 1435-2384 of D16217. Insert lengths(kb): 0.949999988079071 Tissue: placenta Gene product: calpastatin [CAST] Target Gene: calpastatin |
| Insert Size (kb) |
0.950 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.0, 1.0; BamHI--5.9; PstI--4.6, 1.35; HindIII--5.4, 0.5; EcoRI/PstI--4.2, 1.0, 0.7. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.13, 0.54; PstI--0.66. This clone expresses a fusion protein of GST and calpastatin (domains 3 and 4 only). This truncated product also differs from calpastatin by a substitution (G->A) at nt 1937 of D16217, resulting in a glycine to glutamate substitution (G592E). |
| References |
Despres N, et al. Detection and expression of a cDNA clone that encodes a polypeptide containing two inhibitory domains of human calpastatin and its recognition by rheumatoid arthritis sera. J. Clin. Invest. 95: 1891-1896, 1995. PubMed: 7706496
|
