国产片一区二区,欧美视频一二区,亚洲精品国产免费

▍最近瀏覽歷史

[清空]

▍更多產(chǎn)品

貨號(hào)：B245025
名稱(chēng)：Acanthamoeba healyi Moura et al.

貨號(hào)：B245024
名稱(chēng)：Saccharomyces cerevisiae Meyen ex E.C. Hansen

貨號(hào)：B245023
名稱(chēng)：Saccharomyces cerevisiae Meyen ex E.C. Hansen

貨號(hào)：B245022
名稱(chēng)：Eutypella vitis (Schweinitz) Cooke emend. Glawe

貨號(hào)：B245021
名稱(chēng)：Streptomyces scabiei (Thaxter) Lambert and Loria

貨號(hào)：B245020
名稱(chēng)：Graphium cuneiferum (Berkeley et Broome) Mason et Ellis

貨號(hào)：B245019
名稱(chēng)：HFBCI28

貨號(hào)：B245018
名稱(chēng)：Septoria passiflorae Sydow, anamorph

貨號(hào)：B245017
名稱(chēng)：Dictyostelium purpureum Olive

貨號(hào)：B245016
名稱(chēng)：Y71

▍聯(lián)系我們

0574-87157013
mingzhoubio@163.com
浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
創(chuàng)e慧谷42號(hào)樓B幢401室

1G8

規(guī)格:

貨期:

編號(hào):B238827

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購(gòu)物車(chē)

在線咨詢收藏

產(chǎn)品名稱(chēng)	1G8
商品貨號(hào)	B238827
Organism	Ictalurus punctatus, channel catfish
Tissue	peripheral blood
Cell Type	lymphocyte B lymphoblast
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	They are reported to secrete moderate levels of IgM in culture [PubMed: 8133033].
Storage Conditions	liquid nitrogen vapor phase
Karyotype	diploid
Derivation	1G8 (ATCC CRL-2756), developed in 1992, and 3B11 (ATCC CRL-2757), developed in 1994, are immortal B cell lines developed by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from two different normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. They are reported to secrete moderate levels of IgM in culture [PubMed: 8133033]. The 1G8 cells express p53 mRNA levels only two to three times higher than comparable levels found in freshly isolated peripheral blood [PubMed: 11687262]. The 1G8 cells express the heat shock protein hsp70 gene in significantly higher levels than comparable levels found in freshly isolated peripheral blood [PubMed: 11687262]. The 1G8 cells constitutively express telomerase [PubMed: 9914913].
Comments	1G8 (ATCC CRL-2756), developed in 1992, and 3B11 (ATCC CRL-2757), developed in 1994, are immortal B cell lines developed by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from two different normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. They are reported to secrete moderate levels of IgM in culture [PubMed: 8133033]. The 1G8 cells express p53 mRNA levels only two to three times higher than comparable levels found in freshly isolated peripheral blood [PubMed: 11687262]. The 1G8 cells express the heat shock protein hsp70 gene in significantly higher levels than comparable levels found in freshly isolated peripheral blood [PubMed: 11687262]. The 1G8 cells constitutively express telomerase [PubMed: 9914913].
Complete Growth Medium	A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine, and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 87.5%; double distilled water, 10%; heat-inactivated Catfish serum, 2.5% (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
Subculturing	Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml. Interval: Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 27.0°C
Name of Depositor	NW Miller
Year of Origin	1992
References	Miller NW, et al. Development and characterization of channel catfish long term B cell lines. J. Immunol. 152: 2180-2189, 1994. PubMed: 8133033 Wilson M, et al. A novel chimeric Ig heavy chain from a teleost fish shares similarities to IgD. Proc. Natl. Acad. Sci. USA 94: 4593-4597, 1997. PubMed: 9114035 Stuge TB, et al. Development and analysis of various clonal alloantigen-dependent cytotoxic cell lines from channel catfish. J. Immunol. 164: 2971-2977, 2000. PubMed: 10706684 Barker K, et al. Immortal and mortal clonal lymphocyte lines from channel catfish: comparison of telomere length, telomerase activity, tumor suppressor and heat shock protein expression. Dev. Comp. Immunol. 26: 45-51, 2002. PubMed: 11687262 Miller N, et al. Functional and molecular characterization of teleost leukocytes. Immunol. Rev. 166: 187-197, 1998. PubMed: 9914913 Shen L, et al. Channel catfish cytotoxic cells: a mini-review. Dev. Comp. Immunol. 26: 141-149, 2002. PubMed: 11696379 Miller NW, et al. Development of leukocyte cell lines from the channel catfish (Ictalurus punctatus). J. Tissue Culture Methods 16: 117-123, 1994.

序號(hào)	文章標(biāo)題	操作
1	ATCC細(xì)胞運(yùn)輸和處理	查看詳情
2	其他海外品牌代理	查看詳情
3	ATCC代理	查看詳情
4	購(gòu)買(mǎi)須知	查看詳情
5	訂購(gòu)流程	查看詳情

梅經(jīng)理	17280875617	1438578920
胡經(jīng)理	13345964880	2438244627
周經(jīng)理	17757487661	1296385441
于經(jīng)理	18067160830	2088210172
沈經(jīng)理	19548299266	2662369050
李經(jīng)理	13626845108	972239479

国产精品成人久久I97成人精品视频在线播放I综合色综合色I福利视频在线看I美女精品网站I久久综合久久伊人

▍最近瀏覽歷史

▍更多產(chǎn)品

▍聯(lián)系我們