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Construct size (kb): 7.199999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-5Zf(+)
Intact vector size: 3.000
Type of vector: phagemid
Vector end: NcoI
Vector end: SalI
Cloning sites:
Polylinker sites:
Features (with orientation and position when available):

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MGAT1
Other gene symbols: GLYT1; MGAT
Gene name: mannosyl (alpha-1,3-)-glycoprotein
beta-1,2-N-acetylglucosaminyltransferase
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5
Tissue: chronic myeloid leukemia
Type of DNA: genomic
Insert 5' end:
Insert 3' end:
Insert size (kb): 4.5
Cross references: DNA Seq. Acc.: M61829

Insert lengths(kb): 4.5

Tissue: chronic myeloid leukemia

Gene product: mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase [MGAT]

Restriction digests of the clone give the following sizes (kb): NcoI/SalI--4.5, 3.0; EcoRI--7.5; PstI--4.25, 2.25, 0.9; SacI--4.4, 1.95, 0.65, 0.4; SmaI--7.5.

The insert contains the following restriction sites (kb from the 5' end): BglI--3.03; BglII--3.1; EcoRI--4.33; PstI--2.2, 2.4, 3.23, 3.28; PvuII--3.21, 3.28, 4.17; SacI--2.43, 2.58, 3.0; SmaI--2.33.

An 0.8 kb PstI fragment of the insert hybridizes to the following fragments of human genomic DNA (kb): EcoRI--4.7; HindIII--7.2; BamHI--10.4; BglII--4.0; StyI--1.3. Detects a 9.0 kb EcoRI fragment of hamster genomic DNA.

Contains a complete exon 2 (2.5 kb) plus upstream and downstream sequences. Exon 2 contains most of the 5' untranslated region, the complete coding sequence (nt 2182-3519 of the sequence record) and the complete 3' untranslated region.

Derived from pHG15 which was constructed by inserting a SalI fragment from an lambdaEMBL3 library into pGEM-5Z. pHG4.5 is a circularized NcoI fragment from pHG15, and thus retains lambdaEMBL3 sequences from SalI to the 5' end of the insert.

The SP6 promoter is adjacent to the 5' end of the insert.