| 產(chǎn)品名稱 |
Bacillus megaterium |
| 商品貨號(hào) |
B81111 |
| 中文名稱 |
巨大芽孢桿菌 |
| 其它保藏中心編號(hào) |
ATCC 14581=[BCRC 10608, CCM 2007, CCUG 1817, CIP 66.20, DSM 32, HAMBI 2018, IAM 13418, JCM 2506, KCTC 3007, LMG 7127, NBRC 15308, NCCB 75016, NCIMB 9376, NCTC 10342, NRIC 1710, NRRL B-14308, VKM B-512] |
| 特征特性 |
Nucleotide (GenBank) : X53048 B.megaterium TEG gene.Nucleotide (GenBank) : X64338 B.megaterium DNA for orf3/2.Nucleotide (GenBank) : X59070 B.megaterium spoOH gene for sigma H factor.Nucleotide (GenBank) : X16610 Bacillus megaterium gene for cytochrome P-450(BM-1).Nucleotide (GenBank) : X61380 B.megaterium nprM gene for Ca dependant exoprotease.Nucleotide (GenBank) : AF047824 Bacillus megaterium beta-galactosidase (mbgA) gene, complete cds.Nucleotide (GenBank) : J04832 B.megaterium cytochrome P-450:NADPH-P-450 reductase gene, complete cds. Lawrence TS, Ford WW. Studies on aerobic spore-bearing non-pathogenic bacteria. Spore-bearing bacteria in milk. J. Bacteriol. 1: 277-320, 1916.J. Gen. Microbiol. 34: 271, 1964.Shaw GC, et al. A 53-base-pair inverted repeat negatively regulates expression of the adjacent and divergently oriented cytochrome P450(BM-1) gene and its regulatory gene, bm1P1, in Bacillus megaterium. J. Bacteriol. 179: 280-283, 1997. PubMed: 8982010Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.VITEK 2 BCL Comprehensive QC Set. bioMerieux. |
| 培養(yǎng)基 |
營(yíng)養(yǎng)瓊脂或營(yíng)養(yǎng)肉湯培養(yǎng)基 |
| 培養(yǎng)溫度 |
30 ℃, 有氧 |
| 凍干物說(shuō)明 |
·凍干首次活化,干粉要全部用完,不能保留,用0.2-0.5m1的培養(yǎng)液或者無(wú)菌水溶解,接種在1-2個(gè)平板上,因凍干菌種處于休眠狀態(tài),請(qǐng)勿接種多個(gè)平板,以免因接種量不足而導(dǎo)致復(fù)蘇不成功； |
| 保存說(shuō)明 |
1、長(zhǎng)期保存的儲(chǔ)存菌株以冷凍干燥保藏為主，特殊要求的菌種可用其它方法進(jìn)行保存，保存期可長(zhǎng)達(dá)幾年至幾十年。2、半儲(chǔ)存菌株以含20%甘油的肉湯或液體石臘封存的半固體瓊脂為主，在0℃以下保存，保存期為3－6個(gè)月。3、應(yīng)用菌株以瓊脂斜面為主，在4℃保存，保存期為1－3個(gè)月。4、菌種的傳代不可超過(guò)6次。視菌種的特性及使用情況，一般儲(chǔ)存菌株每5年傳記代一次，每1-2年從儲(chǔ)存菌株轉(zhuǎn)接至半儲(chǔ)存菌株，半儲(chǔ)存菌株每3-6個(gè)月傳代一次，每1-3個(gè)月從半儲(chǔ)存菌株轉(zhuǎn)接接至應(yīng)用菌株。 |
